Microfluidic platform for continuous monitoring of gasotransmitters for early level management of Systemic Inflammatory Response Syndrome (SIRS) in Trauma patients
Primary Information
Domain
Environmental Science & Climate Change
Project No.
6183
Sanction and Project Initiation
Sanction No: F.NO.35-16/2016-T.S.-I
Sanction Date: 13/02/2017
Project Initiation date: 19/08/2017
Project Duration: 36 months
Partner Ministry/Agency/Industry
IIT Madras
Role of partner: Share funding with MHRD.
Support from partner: 50% funding.
Principal Investigator
Dr. Ashis Kumar Sen
Indian Institute of Technology, Madras
Co-PIs
Dr. Madhulika Dixit
Indian Institute of Technology, Madras
Paul Ramesh Thangaraj
Apollo Hospitals, Chennai
Muraleedharan K. M.
Indian Institute of technology Madras
Jayashree Gopal
Apollo Hospitals, Chennai
Anil Tarigopula
Apollo Hospitals, Chennai
Anil Prabhakar
Indian Institute of Technology, Madras
Dr. Ananth Krishnan
Indian Institute of Technology, Madras
Host Institute
Scope and Objectives
To develop a microfluidics based LOC platform for continuous monitoring of gasotransmitters in SIRS.
To synthesize stable, sensitive and specific fluorescent probes for gasotransmitters.
To design an optofluidic platform to achieve a fast online concentration gradient of gasotransmitter.
To validate the platform in clinical set-up with known markers of endothelial dysfunction and multiple organ failure.
To compare existing empirical risk scores with the device for outcome predictions.
Deliverables
Continuous on-line monitoring system for early detection of SIRS.
Efficient device for fast, selective and accurate detection of gasotransmitters.
Correlation of relationship between gasotransmitters.
Optofluidic detection setup developed for fast detection.
Efficient LOC for plasma separation.
Predictive algorithms for prognosis of sepsis in SIRS.
Scientific Output
A. Microfluidics platform development
1. Developed a microfluidics platform for continuous monitoring of gasotransmitters.
(B) Probe synthesis
1.Synthesized a stable, sensitive and specific fluorescent probes for fluorescence based detection of H2S.
2. Probes for H2O2,NO and CO have been identified, synthesis in progress.
(C) Clinical trial
1. Platform was used at Apollo Hospitals, Chennai (our collaborator) for two months (March to April 2018) as part of a pilot study to detect the H2S level in ICU patients before, during and after surgery.
Results and outcome till date
A. Patent:
1. Opto-microfluidic device for real-time detection of gases in biosamples and liquids, PCT/IN2018/050194.
(B) Journal paper:
1.Rapid measurement of hydrogen sulphide in human blood plasma using an opto microfluidic device, R. Karunya, K. S. Jayaprakash, R. Gaikwad, P. Sajeesh, K.
2. Ramshad, K. M. Muraleedharan, M. Dixit, P. R. Thangaraj and A. K. Sen, submitted to Scientific Reports (2018).
3. S. Karthick, A. K. Sen, Improved understanding of acoustophoresis and development of an acoustouidic device for blood plasma separation, Phys. Rev. Applied, Accepted (2018).
Societal benefit and impact anticipated
Reports state that 1in 4 ICU patients suffers from sepsis, and 1 in 2 dies eventually15 which indicates that SIRS underlies global medical emergencies. To improve the chances of patient's survival, sepsis must be treated rapidly and efficiently. Every hour of delay in treatment reduces the average patient's survival by 8%. Developing a fast and continuous monitoring device for SIRS detection will bring a huge breakthrough in clinical world for sepsis diagnosis.
Platform was used at Apollo Hospitals, Chennai (our collaborator) for two months (March- April 2018) as part of a pilot study to detect the H2S level in ICU patients before, during and after surgery.
Next steps
Development of the opto- microfluidic platform for detection of H2O2 in blood- in progress.
Continue with the pilot study for measuring H2S levels in ICU patients.
To validate the H2S detection platform in clinical setup with known markers of endothelial dysfunction.
Publications and reports
Rapid measurement of hydrogen sulphide in human blood plasma using an opto microfluidic device, R. Karunya, K. S. Jayaprakash, R. Gaikwad, P. Sajeesh, K.
Ramshad, K. M. Muraleedharan, M. Dixit, P. R. Thangaraj and A. K. Sen, submitted to Scientific Reports (2018).
S. Karthick, A. K. Sen, Improved understanding of acoustophoresis and development of an acoustouidic device for blood plasma separation, Phys. Rev. Applied, Accepted (2018).
Patents
Opto-microfluidic device for real-time detection of gases in biosamples and liquids, PCT/IN2018/050194.
Scholars and Project Staff
2 PDF, 2 PhDs, 6 Project staffs.
Challenges faced
Clinical test requires significant effort and time.
Other information
Financial Information
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Total sanction: Rs. 386.00 lakhs
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Amount received: Rs. 289.00 lakhs
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Amount utilised for Equipment: 130.00 lakhs
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Amount utilised for Manpower: Rs. 90.00 lakhs
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Amount utilised for Consumables: 270.00 lakhs
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Amount utilised for Contingency: 7.00 lakhs
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Amount utilised for Travel: Rs. 5.00 lakhs
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Amount utilised for Other Expenses: Rs. 0.00 lakhs
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Amount utilised for Overheads: 46.00 lakhs
Equipment and facilities
Olympus Inverted Microscope + HS Camera.
Pulsation-free syringe pump.